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Human IL-5 R alpha Protein, His Tag
Catalog Number: orb257612
| Catalog Number | orb257612 |
|---|---|
| Category | Proteins |
| Description | Human IL-5 R alpha, His Tag is expressed from human 293 cells (HEK293). It contains AA Asp 21 - Glu 335 (Accession # NP_000555). |
| Target | IL-5 R alpha |
| Conjugation | Unconjugated |
| Tag | C-10×His |
| Reactivity | Human |
| Form/Appearance | Powder |
| Buffer/Preservatives | PBS, pH 7.4 |
| Purity | 90% |
| Protein Sequence | NP_000555 |
| UniProt ID | Q01344 |
| MW | 37.7 kDa |
| Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 37.7 kDa. The protein migrates as 55-60 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Expression System | HEK293 |
| Endotoxins | 1.0 EU per μg |
| Biological Origin | Human |
| Biological Activity | Loaded Biotinylated Human IL-5, His, (Cat. No. IL5-H82Q5) on SA Biosensor, can bind Human IL-5 R alpha, His Tag (Cat. No. ) with an affinity constant of 12.6 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested). |
| Expression Region | Asp 21 - Glu 335 |
| Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
| Note | For research use only |
| NCBI | NP_000555 |
SDS-PAGE analysis of Human IL-5 R alpha protein
Loaded Biotinylated Human IL-5, His, Avitag on SA Biosensor, can bind Human IL-5 R alpha, His Tag (Cat. No. orb257612) with an affinity constant of 12.6 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).
- Item 1 of 2
Human IL5RA Protein, His Tag [orb689433]
The purity of the protein is greater than 90% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 37.0 kDa after removal of the signal peptide.The apparent molecular mass of IL5RA-His is approximately 50-55 kDa due to glycosylation.
Mammalian
10 μg, 50 μg, 100 μg 
Recombinant Human IL-5 R alpha/CD125 Protein, His Tag [orb1550877]
> 95% by SDS-PAGE.
Recombinant Human IL-5 R alpha/CD125 Protein is produced by Human Cell expression system. The target protein is expressed with sequence (Asp21-Glu335) of human IL-5 R alpha/CD125 (Accession #Q01344) fused with a 6��His Tag at the C-terminus.
50 μgIL-5R alpha/CD125 Protein, Human, Recombinant (His & Avi), Biotinylated [orb1960616]
98.00%
38.7 kDa (predicted). Due to glycosylation, the protein migrates to 55-60 kDa based on Tris-Bis PAGE result.
1 mg, 500 μg, 100 μgIL-5R alpha/CD125 Protein, Human, Recombinant (His & Avi) [orb1960617]
98.00%
38.7 kDa (predicted). Due to glycosylation, the protein migrates to 50-68 kDa based on Tris-Bis PAGE result.
1 mg, 500 μg, 100 μg
