Human IL-27 Ra Protein, Fc Tag

• Catalog Number: orb257609
• Category: Proteins
• Description: Human IL-27 Ra, Fc Tag is expressed from human 293 cells (HEK293). It contains AA Gln 33 - Arg 514 (Accession # AAH28003).
• Target: IL-27 Ra
• Conjugation: Unconjugated
• Tag: C-Fc
• Reactivity: Human
• Form/Appearance: Powder
• Buffer/Preservatives: 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH 7.5
• Purity: 95%
• Protein Sequence: AAH28003
• UniProt ID: Q6UWB1
• MW: 78.7 kDa
• Application notes: This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 78.7 kDa. The protein migrates as 100-116 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expression System: HEK293
• Endotoxins: 1.0 EU per μg
• Biological Origin: Human
• Biological Activity: Human IL-27 Ra Protein, Fc Tag (Cat. No. ) immobilized on CM5 Chip can bind Human IL-27 Protein, Fc Tag (Cat. No. IL7-H5254) with an affinity constant of 3.67 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
• Expression Region: Gln 33 - Arg 514
• Storage: -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
• Note: For research use only
• NCBI: AAH28003

SDS-PAGE analysis of Human IL-27 Ra protein

Human IL-27 Ra Protein, Fc Tag (Cat. No. orb257609) immobilized on CM5 Chip can bind Human IL-27 Protein, Fc Tag with an affinity constant of 3.67 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).