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Human IL-21 R Protein, His Tag
Catalog Number: orb257627
| Catalog Number | orb257627 |
|---|---|
| Category | Proteins |
| Description | Human IL-21 R, His Tag is expressed from human 293 cells (HEK293). It contains AA Cys 20 - Pro 236 (Accession # Q9HBE5-1). |
| Target | IL-21 R |
| Conjugation | Unconjugated |
| Tag | C-10×His |
| Reactivity | Human |
| Form/Appearance | Powder |
| Buffer/Preservatives | PBS, pH 7.4 |
| Purity | 90% |
| Protein Sequence | NP_068570.1 |
| UniProt ID | Q9HBE5 |
| MW | 26.9 kDa |
| Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 26.9 kDa. The protein migrates as 37-47 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Expression System | HEK293 |
| Endotoxins | 1.0 EU per μg |
| Biological Origin | Human |
| Biological Activity | Immobilized Biotinylated Human IL-21, Fc, (Cat. No. IL1-H82F7) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human IL-21 R, His Tag (Cat. No. ) with a linear range of 0.8-3 ng/mL (QC tested). |
| Expression Region | Cys 20 - Pro 236 |
| Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
| Note | For research use only |
| NCBI | NP_068570.1 |
SDS-PAGE analysis of Human IL-21 R protein
Immobilized Biotinylated Human IL-21, Fc, Avitag at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. orb668907) precoated (0.5 μg/well) plate can bind Human IL-21 R, His Tag (Cat. No. orb257627) with a linear range of 0.8-3 ng/mL (QC tested).
