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Catalog Number | orb257628 |
---|---|
Category | Proteins |
Description | Human IL-21 R, Fc Tag is expressed from human 293 cells (HEK293). It contains AA Cys 20 - Pro 236 (Accession # Q9HBE5-1). |
Target | IL-21 R |
Conjugation | Unconjugated |
Tag | C-Fc |
Reactivity | Human |
Form/Appearance | Powder |
Buffer/Preservatives | PBS, pH 7.4 |
Purity | 95% |
Protein Sequence | NP_068570.1 |
UniProt ID | Q9HBE5 |
MW | 51.5 kDa |
Application notes | This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 51.5 kDa. The protein migrates as 65-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expression System | HEK293 |
Endotoxins | 1.0 EU per μg |
Biological Origin | Human |
Biological Activity | Human IL-21, Tag Free (Cat. No. IL1-H5213) immobilized on CM5 Chip can bind Human IL-21 R, Fc Tag (Cat. No. ) with an affinity constant of 0.412 nM as determined in a SPR assay (Biacore 8K) (Routinely tested). |
Expression Region | Cys 20 - Pro 236 |
Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
Note | For research use only |
NCBI | NP_068570.1 |
SDS-PAGE analysis of Human IL-21 R protein
Human IL-21, Tag Free (Cat. No. orb752328) immobilized on CM5 Chip can bind Human IL-21 R, Fc Tag (Cat. No. orb257628) with an affinity constant of 0.412 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).