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Human IL-1RL1 Protein, Fc Tag
Catalog Number: orb257583
| Catalog Number | orb257583 |
|---|---|
| Category | Proteins |
| Description | Human IL-1RL1, Fc Tag is expressed from human 293 cells (HEK293). It contains AA Lys 19 - Pro 323 (Accession # NP_003847). |
| Target | IL-1RL1 |
| Conjugation | Unconjugated |
| Tag | C-Fc |
| Reactivity | Human |
| Form/Appearance | Powder |
| Buffer/Preservatives | Tris with Glycine, Arginine and NaCl, pH 7.5 |
| Purity | 95% |
| Protein Sequence | NP_003847 |
| UniProt ID | Q01638 |
| MW | 61.0 kDa |
| Application notes | This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 61.0 kDa. The protein migrates as 80-100 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Expression System | HEK293 |
| Endotoxins | 1.0 EU per μg |
| Biological Origin | Human |
| Biological Activity | Loaded Human IL-1RL1, Fc Tag (Cat. No. ) on Protein A Biosensor, can bind with Biotinylated Human IL-33, His, (Cat. No. IL3-H82H5) an affinity constant of 1.81 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested). |
| Expression Region | Lys 19 - Pro 323 |
| Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
| Note | For research use only |
| NCBI | NP_003847 |
SDS-PAGE analysis of Human IL-1RL1 protein
Loaded Human IL-1RL1, Fc Tag (Cat. No. orb257583) on Protein A Biosensor, can bind with Biotinylated Human IL-33, His, Avitag an affinity constant of 1.81 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
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