Human IL-18BP Protein, Fc Tag

• Catalog Number: orb257623
• Category: Proteins
• Description: Human IL-18BP, Fc Tag is expressed from human 293 cells (HEK293). It contains AA Thr 31 - Gly 194 (Accession # NP_001138529).
• Reactivity: Human
• Tag: C-Fc
• Form/Appearance: Powder
• Purity: 95%
• Conjugation: Unconjugated
• MW: 44.3 kDa
• Target: IL-18BP
• UniProt ID: O95998
• Source: Human IL-18BP, Fc Tag (orb257623) is expressed from human 293 cells (HEK293). It contains AA Thr 31 - Gly 194 (Accession # NP_001138529).
• Expression System: HEK293
• Biological Origin: Human
• Biological Activity: Human IL-18BP, Fc Tag captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human IL-18, Tag Free with an affinity constant of 1.71 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
• Expression Region: Thr 31 - Gly 194
• Endotoxins: 1.0 EU per μg
• NCBI: NP_001138529
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: Tris with Glycine, Arginine and NaCl, pH7.5
• Alternative names: IL18BP,IL18BPa,Tadekinig-alfa
• Note: For research use only
• Application notes: This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 44.3 kDa. The protein migrates as 55-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

SDS-PAGE analysis of Human IL-18BP protein

Human IL-18BP, Fc Tag (Cat. No. orb257623) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human IL-18, Tag Free with an affinity constant of 1.71 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).