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Human IL-17F Protein, His Tag
Catalog Number: orb334906
| Catalog Number | orb334906 |
|---|---|
| Category | Proteins |
| Description | Human IL-17F (H161R), His Tag is expressed from human 293 cells (HEK293). It contains AA Arg 31 - Gln 163 (Accession # Q96PD4-1 (H161R)). |
| Target | IL-17F |
| Conjugation | Unconjugated |
| Tag | N-6×His |
| Reactivity | Human |
| Form/Appearance | Powder |
| Buffer/Preservatives | PBS, pH 7.4 |
| Purity | 95% |
| Protein Sequence | AAH70124.1 |
| UniProt ID | Q96PD4 |
| MW | 16.8 kDa |
| Application notes | This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 16.8 kDa. The protein migrates as 20-23 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Expression System | HEK293 |
| Endotoxins | 1.0 EU per μg |
| Biological Origin | Human |
| Biological Activity | Loaded Human IL-17 RA, Fc Tag (Cat. No. ILA-H5257) on Protein A Biosensor, can bind Human IL-17F (H161R), His Tag (Cat. No. ) with an affinity constant of 299 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested). |
| Expression Region | Arg 31 - Gln 163 |
| Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
| Note | For research use only |
| NCBI | AAH70124.1 |
SDS-PAGE analysis of Human IL-17F protein
Loaded Human IL-17 RA, Fc Tag on Protein A Biosensor, can bind Human IL-17F (H161R), His Tag (Cat. No. orb334906) with an affinity constant of 299 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
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Human IL17F Protein, His Tag [orb1471791]
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 15.7 kDa after removal of the signal peptide. The apparent molecular mass of His-IL17F is approximately 15-25 kDa due to glycosylation.
Mammalian
100 μg, 10 μg, 50 μg 
Biotinylated Recombinant Human IL-17A&IL-17F heterodimer Protein, Avi His Tag [orb1550461]
> 95% by Tris-Bis PAGE
Biotinylated Recombinant Human IL-17A&IL-17F heterodimer Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Gly24-Ala155(IL-17A)&Arg31-Gln163) of Human IL-17A&IL-17F heterodimer fused with His Tag and Avi tag at the C-terminal.
100 μg
