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Catalog Number | orb257580 |
---|---|
Category | Proteins |
Description | Human IL-13 R alpha 1 Protein, His Tag is expressed from human 293 cells (HEK293). It contains AA Gly 22 - Thr 343 (Accession # NP_001551.1). |
Target | IL-13 R alpha 1 |
Conjugation | Unconjugated |
Tag | C-6×His |
Reactivity | Human |
Form/Appearance | Powder |
Buffer/Preservatives | PBS, pH 7.4 |
Purity | 90% |
Protein Sequence | NP_001551.1 |
UniProt ID | P78552 |
MW | 37.7 kDa |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 37.7 kDa. The protein migrates as 45-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expression System | HEK293 |
Endotoxins | 1.0 EU per μg |
Biological Origin | Human |
Biological Activity | Human IL-13 R alpha 1 Protein, His Tag (Cat. No. ) immobilized on CM5 Chip can bind Human IL-13 Protein, His Tag, premium grade (Cat. No. IL3-H52H4) with an affinity constant of 46.2 nM as determined in a SPR assay (Biacore 8K) (QC tested). |
Expression Region | Gly 22 - Thr 343 |
Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
Note | For research use only |
NCBI | NP_001551.1 |
SDS-PAGE analysis of Human IL-13 R alpha 1 protein
Human IL-13 R alpha 1 Protein, His Tag (Cat. No. orb257580) immobilized on CM5 Chip can bind Human IL-13 Protein, His Tag, premium grade (Cat. No. orb612135) with an affinity constant of 46.2 nM as determined in a SPR assay (Biacore 8K) (QC tested).
Unconjugated | |
95% | |
50.2 kDa & 63.1 kDa |
> 95% by SDS-PAGE. | |
Recombinant Cynomolgus PD-1 Protein is produced by mammalian expression system. The target protein is expressed with sequence (Ala27-Thr343) of human IL-13 R alpha 1 (Accession #P78552) fused with a 6xHis Tag, Avi tag at the C-terminus. |