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Human IL-12B Protein, His Tag
Catalog Number: orb257709
| Catalog Number | orb257709 |
|---|---|
| Category | Proteins |
| Description | Human IL-12B, His Tag is expressed from human 293 cells (HEK293). It contains AA Ile 23 - Ser 328 (Accession # P29460-1). |
| Target | IL-12B |
| Conjugation | Unconjugated |
| Tag | C-10×His |
| Reactivity | Human |
| Form/Appearance | Powder |
| Buffer/Preservatives | PBS, pH 7.4 |
| Purity | 95% |
| Protein Sequence | AAH67499.1 |
| UniProt ID | P29460 |
| MW | 36.6 kDa |
| Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 36.6 kDa. The protein migrates as 44 kDa and 46 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Expression System | HEK293 |
| Endotoxins | 1.0 EU per μg |
| Biological Origin | Human |
| Biological Activity | Loaded Human IL-12 R beta 1, Fc Tag (Cat. No. ILB-H5255) on Protein A Biosensor, can bind Human IL-12B, His Tag (Cat. No. ) with an affinity constant of 3.78 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested). |
| Expression Region | Ile 23 - Ser 328 |
| Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
| Note | For research use only |
| NCBI | AAH67499.1 |
SDS-PAGE analysis of Human IL-12B protein
Loaded Human IL-12 R beta 1, Fc Tag (Cat. No. orb570282) on Protein A Biosensor, can bind Human IL-12B, His Tag (Cat. No. orb257709) with an affinity constant of 3.78 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
- Item 1 of 1
Human IL12A and IL12B Heterodimer Protein, hFc Tag and His Tag [orb1743293]
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 48.7 and 35.5 kDa after removal of the signal peptide.
Mammalian
50 μg, 10 μg, 100 μg - Item 1 of 1
Human IL23A and IL12B Heterodimer Protein, hFc Tag and His Tag [orb1743335]
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 44.8 and 35.5 kDa after removal of the signal peptide. The apparent molecular mass of IL23A-hFc and IL12B-His is approximately 35-55 kDa due to glycosylation.
E.coli
10 μg, 50 μg, 100 μg - Item 1 of 1
Human IL12B Protein, His Tag [orb1290821]
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 35.5 kDa after removal of the signal peptide. The apparent molecular mass of IL12B-His is approximately 35-55 kDa due to glycosylation.
Mammalian
100 μg, 10 μg, 50 μg 
Human IL12A & IL27B Protein, Flag & His Tag [orb2698041]
> 90%, determined by SDS-PAGE
This protein contains the human IL12A (NP_0008732)(Met1-Ser219) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; This protein contains the human IL27B (NP_0057462)(Met1-Lys229) was fused with a flag tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the human IL27B&IL12A heterodimer was purified and expressed from HEK293 Cells.
100 μg, 20 μgHuman IL12A & IL12B Protein, His Tag [orb2698050]
> 95%, determined by SDS-PAGE
This protein contains the human IL12 complex composed of IL12B (NP_0021782) ( Met1-Ser328) and the mature form of human IL12A ( NP_0008732)( Arg 23 - Ser 219 ) linked by a polypeptide linker was fused with a polyhistidine tag at the C-terminus and expressed from HEK293 Cells.
20 μg, 5 μg
