Human IL-1 RII Protein, His Tag

• Catalog Number: orb257591
• Category: Proteins
• Description: Human IL-1 RII, His Tag is expressed from human 293 cells (HEK293). It contains AA Phe 14 - Glu 343 (Accession # NP_004624.1).
• Target: IL-1 RII
• Conjugation: Unconjugated
• Tag: C-8×His
• Reactivity: Human
• Form/Appearance: Powder
• Buffer/Preservatives: PBS, pH 7.4
• Purity: 98%
• Protein Sequence: NP_004624.1
• UniProt ID: P27930
• MW: 38.9 kDa
• Application notes: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 38.9 kDa. The protein migrates as 50-60 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expression System: HEK293
• Endotoxins: 1.0 EU per μg
• Biological Origin: Human
• Biological Activity: Immobilized Human IL-1 beta, Fc Tag (Cat. No. ILA-H525c) at 5 μg/mL (100 μL/well) can bind Human IL-1 RII, His Tag (Cat. No. ) with a linear range of 1-20 ng/mL (QC tested).
• Expression Region: Phe 14 - Glu 343
• Storage: -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
• Note: For research use only
• NCBI: NP_004624.1

SDS-PAGE analysis of Human IL-1 RII protein

Immobilized Human IL-1 beta, Fc Tag (Cat. No. orb752327) at 5 μg/mL (100 μL/well) can bind Human IL-1 RII, His Tag (Cat. No. orb257591) with a linear range of 1-20 ng/mL (QC tested).