Human IL-1 RII Protein, Fc Tag

• Catalog Number: orb257592
• Category: Proteins
• Description: Human IL-1 RII, Fc Tag is expressed from human 293 cells (HEK293). It contains AA Phe 14 - Glu 343 (Accession # NP_004624.1).
• Target: IL-1 RII
• Conjugation: Unconjugated
• Tag: C-Fc
• Reactivity: Human
• Form/Appearance: Powder
• Buffer/Preservatives: 50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH 7.5
• Purity: 95%
• UniProt ID: NP_004624.1
• MW: 63.9 kDa
• Application notes: This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 63.9 kDa. The protein migrates as 70 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expression System: HEK293
• Endotoxins: 1.0 EU per μg
• Expression Region: Phe 14 - Glu 343
• Storage: -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
• Research Area: Disease Biomarkers
• Note: For research use only
• Expiration Date: 6 months from date of receipt.

SDS-PAGE analysis of Human IL-1 RII protein

Line graph illustrates about the sensitivity range of Human IL-1 RII protein using different concentrations

Human IL-1 RII, Fc Tag (Cat. No. orb257592) captured on CM5 chip via anti-human IgG Fc antibodies surface can bind Human IL-1 beta, premium grade (Cat. No. orb257615) with an affinity constant of 0.622 nM as determined in a SPR assay (Biacore T200) (Routinely tested).