Human IGFBP-7 Protein, His Tag

• Catalog Number: orb348791
• Category: Proteins
• Description: Human IGFBP-7, His Tag is expressed from human 293 cells (HEK293). It contains AA Asp 30 - Leu 282 (Accession # NP_001544).
• Target: IGFBP-7
• Conjugation: Unconjugated
• Tag: N-6×His
• Reactivity: Human
• Form/Appearance: Powder
• Buffer/Preservatives: PBS, pH 7.4
• Purity: 95%
• Protein Sequence: NP_001544
• UniProt ID: Q16270
• MW: 27.0 kDa
• Application notes: This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 27.0 kDa. The protein migrates as 33-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expression System: HEK293
• Endotoxins: 1.0 EU per μg
• Biological Origin: Human
• Biological Activity: Human IGFBP-7, His Tag (Cat. No. ) immobilized on CM5 Chip can bind Human C1q R1, His Tag (Cat. No. C11-H5228) with an affinity constant of 16.7 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).
• Expression Region: Asp 30 - Leu 282
• Storage: -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
• Note: For research use only
• NCBI: NP_001544

SDS-PAGE analysis of Human IGFBP-7 protein

Human IGFBP-7, His Tag (Cat. No. orb348791) immobilized on CM5 Chip can bind Human C1q R1, His Tag (Cat. No. orb257231) with an affinity constant of 16.7 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).