Human IGF-II Protein, Fc Tag

• Catalog Number: orb257566
• Category: Proteins
• Description: Human IGF-II, Fc Tag is expressed from human 293 cells (HEK293). It contains AA Ala 25 - Glu 91 (Accession # P01344-1).
• Target: IGF-II
• Conjugation: Unconjugated
• Tag: N-Fc
• Reactivity: Human
• Form/Appearance: Powder
• Buffer/Preservatives: 50 mM Tris, 100 mM Glycine, pH 7.5
• Purity: 95%
• Protein Sequence: P01344-1
• UniProt ID: P01344
• MW: 34.4 kDa
• Application notes: This protein carries a human IgG1 Fc tag at the N-terminus. The protein has a calculated MW of 34.4 kDa. The protein migrates as 35-38 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expression System: HEK293
• Endotoxins: 1.0 EU per μg
• Biological Origin: Human
• Biological Activity: Immobilized Human IGFBP-3, His Tag (Cat. No. IG3-H52H9) at 0.5 μg/mL (100 μL/well) can bind Human IGF-II, Fc Tag (Cat. No. ) with a linear range of 1.2-39 ng/mL (QC tested).
• Expression Region: Ala 25 - Glu 91
• Storage: -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
• Note: For research use only
• NCBI: P01344

SDS-PAGE analysis of Human IGF-II protein

Immobilized Human IGFBP-3, His Tag (Cat. No. orb1146966) at 0.5 μg/mL (100 μL/well) can bind Human IGF-II, Fc Tag (Cat. No. orb257566) with a linear range of 1.2-39 ng/mL (QC tested).