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Human IFN-alpha / beta R2 Protein, Fc Tag
Catalog Number: orb257561
| Catalog Number | orb257561 |
|---|---|
| Category | Proteins |
| Description | Human IFNAR2, Fc Tag is expressed from human 293 cells (HEK293). It contains AA Ile 27 - Lys 243 (Accession # P48551-2). |
| Target | IFN-alpha/beta R2 |
| Conjugation | Unconjugated |
| Tag | C-Fc |
| Reactivity | Human |
| Form/Appearance | Powder |
| Buffer/Preservatives | Tris with Glycine, Arginine and NaCl, pH 7.5 |
| Purity | 95% |
| Protein Sequence | NP_997467.1 |
| UniProt ID | P48551 |
| MW | 51.4 kDa |
| Application notes | This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 51.4 kDa. The protein migrates as 66-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Expression System | HEK293 |
| Endotoxins | 1.0 EU per μg |
| Biological Origin | Human |
| Biological Activity | Loaded Human IFN-alpha 1, His Tag (Cat. No. IFA-H52H9) on HIS1K Biosensor, can bind Human IFNAR2, Fc Tag (Cat. No. ) with an affinity constant of 19.5 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested). |
| Expression Region | Ile 27 - Lys 243 |
| Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
| Note | For research use only |
| NCBI | NP_997467.1 |
SDS-PAGE analysis of Human IFN-alpha/beta R2 protein
Loaded Human IFN-alpha 1, His Tag (Cat. No. orb735041) on HIS1K Biosensor, can bind Human IFNAR2, Fc Tag (Cat. No. orb257561) with an affinity constant of 19.5 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
