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Human IFN-alpha / beta R2 Protein, His Tag
Catalog Number: orb257560
| Catalog Number | orb257560 |
|---|---|
| Category | Proteins |
| Description | Human IFNAR2, His Tag is expressed from human 293 cells (HEK293). It contains AA Ile 27 - Lys 243 (Accession # P48551-2). |
| Target | IFN-alpha/beta R2 |
| Conjugation | Unconjugated |
| Tag | C-8×His |
| Reactivity | Human |
| Form/Appearance | Powder |
| Buffer/Preservatives | PBS, pH 7.4 |
| Purity | 95% |
| Protein Sequence | NP_997467.1 |
| UniProt ID | P48551 |
| MW | 25.8 kDa |
| Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 25.8 kDa. The protein migrates as 34-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Expression System | HEK293 |
| Endotoxins | 1.0 EU per μg |
| Biological Origin | Human |
| Biological Activity | Human IFN-alpha 2b Protein, Fc Tag (Cat. No. IFB-H5253) immobilized on CM5 Chip can bind Human IFNAR2, His Tag (Cat. No. ) with an affinity constant of 0.257 μM as determined in SPR assay (Biacore T200) (Routinely tested). |
| Expression Region | Ile 27 - Lys 243 |
| Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
| Note | For research use only |
| NCBI | NP_997467.1 |
SDS-PAGE analysis of Human IFN-alpha/beta R2 protein
Human IFN-alpha 2b Protein, Fc Tag immobilized on CM5 Chip can bind Human IFNAR2, His Tag (Cat. No. orb257560) with an affinity constant of 0.257 μM as determined in SPR assay (Biacore T200) (Routinely tested).
