Human Gremlin Protein, His Tag

• Catalog Number: orb18284
• Category: Proteins
• Description: Human Gremlin, His Tag is expressed from human 293 cells (HEK293). It contains AA Lys 25 - Asp 184 (Accession # NP_037504).
• Target: Gremlin
• Conjugation: Unconjugated
• Tag: C-10×His
• Reactivity: Human
• Form/Appearance: Powder
• Buffer/Preservatives: 10 mM Sodium Citrate, pH 3.0
• Purity: 95%
• UniProt ID: NP_037504
• RRID: AB_10751709
• MW: 20.2 kDa
• Application notes: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 20.2 kDa. The protein migrates as 28-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expression System: HEK293
• Endotoxins: 1.0 EU per μg
• Expression Region: Lys 25 - Asp 184
• Storage: -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
• Research Area: Immunology & Inflammation
• Note: For research use only
• Expiration Date: 6 months from date of receipt.

SDS-PAGE analysis of Human Gremlin protein

Immobilized Human BMP-2, Tag Free (Cat. No. orb257227) at 2 μg/mL (100 μL/well) can bind Human Gremlin, His Tag (Cat. No. orb18284) with a linear range of 0.02-0.313 μg/mL (QC tested).