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Catalog Number | orb257498 |
---|---|
Category | Proteins |
Description | Human G-CSF R, Fc Tag is expressed from human 293 cells (HEK293). It contains AA Glu 25 - Pro 621 (Accession # NP_000751.1). |
Target | G-CSF R |
Conjugation | Unconjugated |
Tag | C-Fc |
Reactivity | Human |
Form/Appearance | Powder |
Buffer/Preservatives | Tris with Glycine, Arginine and NaCl, pH 7.5 |
Purity | 95% |
Protein Sequence | NP_000751.1 |
UniProt ID | Q99062 |
MW | 92.7 kDa |
Application notes | This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 92.7 kDa. The protein migrates as 100-120 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expression System | HEK293 |
Endotoxins | 1.0 EU per μg |
Biological Origin | Human |
Biological Activity | Immobilized Human G-CSF Protein, premium grade (Cat. No. GCF-H5214) at 2 μg/mL (100 μL/well) can bind Human G-CSF R, Fc Tag (Cat. No. ) with a linear range of 1-16 ng/mL (QC tested). |
Expression Region | Glu 25 - Pro 621 |
Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
Note | For research use only |
NCBI | NP_000751.1 |
SDS-PAGE analysis of Human G-CSF R protein
Immobilized Human G-CSF Protein, premium grade (Cat. No. orb257496) at 2 μg/mL (100 μL/well) can bind Human G-CSF R, Fc Tag (Cat. No. orb257498) with a linear range of 1-16 ng/mL (QC tested).