Human Dkk-1 Protein, His Tag

• Catalog Number: orb257395
• Category: Proteins
• Description: Human Dkk-1 Protein, His Tag, premium grade is expressed from human 293 cells (HEK293). It contains AA Thr 32 - His 266 (Accession # NP_036374.1).
• Target: Dkk-1
• Conjugation: Unconjugated
• Tag: C-6×His
• Reactivity: Human
• Form/Appearance: Powder
• Buffer/Preservatives: PBS, pH 7.4
• Purity: 95%
• UniProt ID: NP_036374.1
• MW: 26.6 kDa
• Application notes: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 26.6 kDa. The protein migrates as 35-45 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expression System: HEK293
• Endotoxins: 0.1 EU per μg
• Expression Region: Thr 32 - His 266
• Storage: -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
• Research Area: Disease Biomarkers
• Note: For research use only
• Expiration Date: 6 months from date of receipt.

References

Li, Chunyan et al. The Effect of C-X-C Motif Chemokine 13 on Hepatocellular Carcinoma Associates with Wnt Signaling Biomed Res Int, 2015, 345413 (2015)

PubMed: 26161394

SDS-PAGE analysis of Human Dkk-1 protein

Immobilized Human Dkk-1 Protein, His Tag, premium grade (Cat. No. orb257395) at 5 μg/mL (100 μL/well) can bind Human LRP-5, Mouse IgG2a Fc Tag (Cat. No. orb570288) with a linear range of 0.01-0.156 μg/mL (QC tested).