Human Dehydroepiandrosterone sulfate (DHEA-S) ELISA Kit

SKU: orb782367

Description

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with DHEA-S(Dehydroepiandrosterone sulfate) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to DHEA-S(Dehydroepiandrosterone sulfate).

Research Area

Metabolism Research, Musculoskeletal & Connective Tissue Research, Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 400 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human DHEA-S protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human DHEA-S. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human DHEA-S in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: Serum, plasma and other biological fluids
• Assay Type: Competitive
• Assay Time: 2h
• Range: 6.25-400 ng/mL
• Sensitivity: 4.44 ng/mL
• Concentration: 400 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

Androstenolone sulfate ELISA Kit, Prasterone sulfate ELISA Kit, Androst-5-en-3β-ol-17-one 3β-sulfate ELISA Kit