Human Dehydroepiandrosterone Sulfate (DHEA-S) ELISA Kit

• Catalog Number: orb1946838
• Category: Assays and Kits
• Description: Human Dehydroepiandrosterone Sulfate (DHEA-S) ELISA Kit
• Target: DHEA-S
• Reactivity: Human
• Application notes: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human DHEA-S. During the reaction, Human DHEA-S in the sample or standard competes with a fixed amount of Human DHEA-S on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human DHEA-S. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human DHEA-S in tested samples can be calculated by comparing the OD of the samples to the standard curve.
• Assay Type: Competitive
• Assay Time: 1.5H
• Range: 15.63-1000ng/mL
• Sensitivity: 9.38ng/mL
• Sample Types: Serum, Plasma, Tissue homogenate and Other biological samples
• Alternative names: Androstenolone sulfate; Prasterone sulfate; Andros
• Note: For research use only