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Human Activin A Protein, His Tag
Catalog Number: orb383533
| Catalog Number | orb383533 |
|---|---|
| Category | Proteins |
| Description | Human Latent Activin A, His Tag is expressed from human 293 cells (HEK293). It contains AA Ser 21 - Ser 426 (Accession # AAH07858.1). |
| Target | Activin A |
| Conjugation | Unconjugated |
| Tag | N-6×His |
| Reactivity | Human |
| Form/Appearance | Powder |
| Buffer/Preservatives | PBS, pH 7.4 |
| Purity | 95% |
| Protein Sequence | AAH07858.1 |
| UniProt ID | P08476 |
| MW | 45.9 kDa |
| Application notes | This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 45.9 kDa. As a result of glycosylation, the protein migrates as 15 kDa and 40-45 kDa under reducing (R) condition, and 24 kDa, 40-45 kDa and 60 kDa under non-reducing (NR) condition (SDS-PAGE). |
| Expression System | HEK293 |
| Endotoxins | 1.0 EU per μg |
| Biological Origin | Human |
| Biological Activity | Immobilized Human Latent Activin A, His Tag (Cat. No. ) at 10 μg/mL (100 μL/well) can bind Human ACVR2A, Fc Tag (Cat. No. ACA-H5269) with a linear range of 0.6-20 ng/mL (QC tested). |
| Expression Region | Ser 21 - Ser 426 |
| Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
| Note | For research use only |
| NCBI | AAH07858.1 |
SDS-PAGE analysis of Human Activin A protein
Immobilized Human Latent Activin A, His Tag (Cat. No. orb383533) at 10 μg/mL (100 μL/well) can bind Human ACVR2A, Fc Tag (Cat. No. orb1101193) with a linear range of 0.6-20 ng/mL (QC tested).
Human ACVR1B Protein, His Tag [orb2701400]
> 92%, determined by SDS-PAGE
This protein contains the human ACVR1B (NP_004293.1) extracellular domain (Met 1-Glu 126) was expressed from HEK293 Cells.
50 μg, 20 μgHuman ACVR2A Protein, His Tag [orb2701709]
> 97%, determined by SDS-PAGE
This protein contains the extracellular domain of human ACVR2A (NP_001607.1) (Met 1-Pro 134) was expressed from HEK293 Cells.
200 μg, 100 μgHuman ACVR2B Protein, His Tag [orb2701731]
> 97%, determined by SDS-PAGE
This protein contains the extracellular domain of human ACVR2B (NP_001097.2) (Met 1-Thr 134) was fused with a polyhistidine tag at the C-terminus and expressed from HEK293 Cells.
200 μg, 100 μgHuman ACVR1/ALK2 Protein, His Tag [orb2701733]
> 93%, determined by SDS-PAGE
This protein contains the extracellular domain (Met 1-Val 124) of human ALK2 (Q04771) (Met 1-Val 124) was fused with a polyhistidine tag at the C-terminus and expressed from Baculovirus-Insect Cells.
100 μg, 200 μgHuman ACVR1/ALK2 Protein, Fc & His Tag [orb2701734]
> 95%, determined by SDS-PAGE
This protein contains (Met 1-Val 124) of human ALK2 (NP_0011045371) precursor was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus and expressed from HEK293 Cells.
200 μg, 100 μg
