HPV16 E7 Rabbit Polyclonal Antibody

• Catalog Number: orb10573
• Category: Antibodies
• Description: HPV16 E7 Rabbit Polyclonal Antibody
• Target: L2
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from HPV16 E7 protein (41-90/98aa)
• RRID: AB_10746667
• MW: 11 kDa
• Tested applications: FC, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: E7; HPV16 E7 protein; HPV 16 E7 protein; Human Pap
• Research Area: HPV, Human Papillomavirus, Infectious Disease
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Hu, Zheng et al. TALEN-mediated targeting of HPV oncogenes ameliorates HPV-related cervical malignancy J Clin Invest, 125, 425-436 (2015)

PubMed: 25500889

Applications

IHC, WB

Reactivity

Mouse

Gao, Chun et al. The application of CRISPR/Cas9 system in cervical carcinogenesis Cancer Gene Ther, (2021)

PubMed: 34349239

Applications

IF, IHC, WB

Reactivity

Mouse

Xiong, Jinfeng et al. E7-Targeted Nanotherapeutics for Key HPV Afflicted Cervical Lesions by Employing CRISPR/Cas9 and Poly (Beta-Amino Ester) Int J Nanomedicine, 16, 7609-7622 (2021)

PubMed: 34819726

Applications

IHC

Reactivity

Mouse

Zhu, Da et al. Nanoparticles Based on Poly (β-Amino Ester) and HPV16-Targeting CRISPR/shRNA as Potential Drugs for HPV16-Related Cervical Malignancy Mol. Ther., 26, 2443-2455 (2018)

PubMed: 30241742

Applications

IF, IHC

Reactivity

Mouse

Li, Mengtao et al. Exposure to microbial metabolite butyrate prolongs the survival time and changes the growth pattern of HPV16 E6/E7-immortalized keratinocytes in vivo Am J Pathol, (2021)

PubMed: 34214507

Applications

IF

Reactivity

Mouse

Zheng Feng , Zhouzhan Luo Therapeutic efficacy of CRISPR RNA nanoparticles on cervical cancer disease using ultrasound elastography imaging Journal of Radiation Research and Applied Sciences, (2024)

PubMed:

Blank control: Hela. Primary Antibody (green line): Rabbit Anti-HPV16 E7 antibody (orb10573), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-PE, Dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: Lane 1: Recombinant HPV16 E7 protein Bacterial lysate, DsbC & His, Primary: Anti-HPV16 E7 (orb10573) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 11 kDa, Observed band size: 61 kDa.