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HDAC3 Antibody
SKU: orb1260861
Featured
Description
Images & Validation
−Item 1 of 7
| Tested Applications | IF, IHC, WB |
|---|---|
| Reactivity | Human, Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 330 to the C-terminus of human HDAC3 (NP_003874.2). |
| Target | HDAC3 |
| Molecular Weight | Observed: 48kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Liquid |
| Concentration | batch dependent |
| Disclaimer | For research use only |
Alternative Names
−HDAC3, RPD3, SMAP45, HD3, Histone deacetylase 3, RPD3-2
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Western blot analysis of extracts of various cell lines, using HDAC3 antibody (orb1260861) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Western blot analysis of extracts of mouse brain, using HDAC3 antibody (orb1260861) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded mouse liver using HDAC3 antibody (orb1260861) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using HDAC3 antibody (orb1260861) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using HDAC3 antibody (orb1260861) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using HDAC3 antibody (orb1260861) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using HDAC3 Polyclonal Antibody (orb1260861) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Quick Database Links
Gene Symbol
HDAC3
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
HDAC3 Antibody (orb1260861)
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