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Catalog Number | orb1925544 |
---|---|
Category | Antibodies |
Description | Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB56167 |
Tested applications | WB |
Isotype | Rabbit IgG |
Dilution range | WB: 1:2000 |
Conjugation | Unconjugated |
Target | This antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide of HA.2x tag. |
UniProt ID | NA |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
All lanes: Anti-HA Tag Antibody at 1:2000 dilution (upper) or GAPDH (lower). Lane 1: 293T/17 transfected with 12tag lysate (10 ug). Lane 2: Non-transfected 293T/17 lysate (10 ug). Lane 3: 12tag recombinant protein lysate (0. 1ug). Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 45-50 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.