UBC3B Antibody

• Catalog Number: orb125001
• Category: Antibodies
• Description: Goat polyclonal antibody to UBE2R2
• Target: UBC3B
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human, Rat
• Predicted Reactivity: Mouse
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: AQQQMTSSQKALM
• MW: 27
• Tested applications: ELISA, IEM, IHC
• Dilution range: ELISA: 1:4000, WB: 1 μg/ml, IHC-P: 2-4µg/ml
• Application notes: WB: No signal obtained yet but low background observed in Human Testis and U937 lysates at up to 1µg/ml. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?. IHC: In paraffin embedded Human Testis shows textured nuclear staining of the primary spermatocytes . Recommended concentration, 2-4µg/ml.. Notes: Immunoelectron microscopy: This product stained chromatoid bodies in Rat spermatids.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti UBE2R2 antibody, anti UBC3B antibody, anti CD
• Note: For research use only
• Entrez: 54926

Immunohistochemical staining of Human Testis using UBE2R2 antibody