CDC34B Antibody

• Catalog Number: orb382107
• Category: Antibodies
• Description: The CDC34B Antibody is suitable for IF, WB. It is a Polyclonal, Unconjugated antibody which raised against Recombinant fusion protein of human CDC34B .Purification: The antibody was purified by immunogen affinity chromatography.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: Recombinant fusion protein of human CDC34B
• UniProt ID: Q6ZWZ2, Q712K3
• Tested applications: IF, WB
• Dilution range: WB: 1:500-2000
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CDC34B; UBC3B; Ubiquitin-conjugating enzyme E2 R2;
• Research Area: Epigenetics
• Note: For research use only
• Entrez: 67615, 54926
• Expiration Date: 12 months from date of receipt.

Western blot analysis of CDC34B expression in Jurkat (A), MCF7 (B), mouse liver (C), mouse kidney (D), rat brain (E), rat lung (F) whole cell lysates. (Predicted band size: 27 kD; Observed band size: 27 kD)

Immunofluorescent analysis of CDC34B staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).