Goat anti-Rat IgG (H&L), HRP conjugated, min, cross-reactivity to human, mouse lgG (highly absorbed against mouse IgG)

• Catalog Number: orb699952
• Category: Antibodies
• Description: Goat polyclonal antibody against Goat anti-Rat IgG (H&L) conjugated to HRP
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: HRP
• Reactivity: Rat
• Form/Appearance: Lyophilized
• Purity: Immunogen affinity purified goat IgG.
• Purification: HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free0.1 % (v/v) of Kathon CG is used as preservative.
• Immunogen: Purified Rat IgG, whole molecule
• Tested applications: ELISA, IHC, WB
• Dilution range: 1 : 10 000 -1 : 50 000 (ELISA), 1 : 500 -1 : 5000 (IHC), 1 : 10 000 -1 : 50 000 (WB)
• Application notes: No reactivity is observed to non-immunoglobulin rat serum, Antibody has been absorbed against mouse IgG and no reactivity is observed to human or mouse IgG based on immunoelectrophoresis
• Storage: Store non-diluted antibody at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.