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| Catalog Number | orb700285 |
|---|---|
| Category | Antibodies |
| Description | Goat polyclonal antibody against Goat anti-Rat IgG (H&L) conjugated to AP |
| Clonality | Polyclonal |
| Species/Host | Goat |
| Conjugation | ALP |
| Reactivity | Rat |
| Form/Appearance | Liquid |
| Purity | Immunogen affinity purified goat IgG. |
| Purification | APL conjugate is supplied in 30 mM Triethanolamine, pH 7,2, 5 mM Magnesium Chloride, 0,1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) of sodium azide is added as preservative |
| Immunogen | Purified Rat IgG, whole molecule |
| Tested applications | ELISA, ICC, IHC, WB |
| Dilution range | 1 : 500-1 :2000 (ELISA), 1 : 50-1 : 5000 (ICC), 1 : 20 -1 : 2000 (IHC), 1 : 500-1 :2000 (WB) |
| Application notes | This antibody is highly cross absorbed against mouse IgG, No reactivity is observed to non-immunoglobulin rat serum proteins based on immunoelectrophoresis, No reactivity is observed to human or mouse IgG based on immunoelecrophoresis, |
| Storage | Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming. |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
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