MAPRE3 (aa151-164) Antibody

• Catalog Number: orb20476
• Category: Antibodies
• Description: Goat polyclonal antibody to EBF3
• Target: MAPRE3 (aa151-164)
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: KLIGTAVPQRTSPT
• RRID: AB_10921830
• MW: 32
• Tested applications: ELISA, WB
• Dilution range: ELISA: 1:16000, WB: 0.1-0.3 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 33kDa band observed in Human Brain (Cerebellum) and Mouse Brain lysates (calculated MW of 32.0kDa according to Human NP_036458.2 and Mouse NP_579928.1). Recommended concentration: 0.05-0.2 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-COE 3 antibody, anti-COE3 antibody, anti-COE3
• Note: For research use only
• Entrez: 22924
• Expiration Date: 12 months from date of receipt.

orb20476 (0.05ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot analysis of Human (Lane1), Mouse (Lane2) and Rat (Lane3) Brain lysate using MAPRE3 antibody