Goat anti-Clusterin / ApoJ (mouse) Antibody

• Catalog Number: orb20050
• Category: Antibodies
• Description: Goat polyclonal antibody to CLU
• Target: Clusterin / ApoJ (mouse)
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Mouse, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: EKALQEYRRKSRAE
• RRID: AB_10749258
• MW: 51.6
• Tested applications: ELISA, IF, WB
• Dilution range: ELISA: 1:64000, WB: 0.1-1 μg/ml, IHC-P: 10 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx. 37kDa band observed in Mouse Brain and Testis lysates and in Rat Brain and Ovary lysates (calculated MW of 51.6kDa according to Mouse NP_038520.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Miyata et al, Circulation. 2001 Sep 18;104(12):1407-12.; PMID: 11560857). Recommended concentration: 0.3-1 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti Clusterin antibody, anti CLU antibody, anti A
• Research Area: Apoptotic, Cancer, Epigenetics, Immunology, Neuros
• Note: For research use only

Western blot analysis of Rat Brain lysate using CLU antibody

Western blot analysis of Rat Brain lysate using CLU antibody

Western blot analysis of Human Testes lysate using CLU antibody

Immunofluorescence analysis of paraformaldehyde fixed HeLa cells using CLU antibody