Annexin I Antibody

• Catalog Number: orb20132
• Category: Antibodies
• Description: Goat polyclonal to Annexin I
• Target: Annexin I
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Porcine, Rat
• Predicted Reactivity: Bovine, Canine
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: CQAILDETKGDYEK
• RRID: AB_10750616
• MW: 38.7
• Tested applications: ELISA, WB
• Dilution range: ELISA: 1:4000, WB: 1-3 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 35kDa band observed in Human lysates of cell lines A431 and K562 (calculated MW of 38.7kDa according to NP_000691.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Paweletz et al , Cancer Res. 2000 Nov15;60(22):6293-7. PMID: 11103786). Recommended concentration: 0.5-1.5 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti annexin I antibody, anti ANXA1 antibody, anti
• Note: For research use only
• Entrez: 301
• Expiration Date: 12 months from date of receipt.

Western blot analysis of A431 lysate using Annexin antibody

Western blot analysis of K562 (Lane 1) and NIH3T3 (Lane 2) lysates using Annexin I antibody

Immunohistochemical staining of Human Placenta using Annexin I antibody

Immunohistochemical staining of Human Prostate using Annexin I antibody

Western blot analysis of Rat Spleen lysate using Annexin I antibody

Western blot analysis of Pig Spleen lysate using Annexin I antibody