Annexin V Rabbit Polyclonal Antibody

• Catalog Number: orb10101
• Category: Antibodies
• Description: Annexin V Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Mouse, Rat
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: recombind human Annexin V
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg /test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 36 kDa
• Target: ANXA5
• UniProt ID: P08758
• RRID: AB_10921987
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Anchorin CI; Annexin 5; Annexin A5; Annexin V; Ann
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control: RSC96 (blue). Primary Antibody: Rabbit Anti-Annexin V antibody (orb10101), Dilution: 1 µg in 100 µL 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol, The cells were fixed with 2% paraformaldehyde (10 min), then permeabilized with 90% ice-cold methanol for 30 min on ice. Antibody (orb10101, 1 µg/1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody of orb10101 at 1/200 dilution for 30 min on ice. Acquisition of 20000 events was performed.

Sample: Lane 1: Mouse Lung tissue lysates, Lane 2: Mouse Placenta tissue lysates, Lane 3: Mouse Uterus tissue lysates, Lane 4: Mouse Spleen tissue lysates, Lane 5: Mouse Stomach tissue lysates, Lane 6: Rat Lung tissue lysates, Lane 7: Rat Placenta tissue lysates, Lane 8: Rat Spleen tissue lysates, Lane 9: Human HepG2 cell lysates, Lane 10: Human Panc-1 cell lysates, Primary: Anti-Annexin V (orb10101) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 36 kD, Observed band size: 36 kD.

Sample: Lung (Mouse) Lysate at 40 ug, Primary: Anti-Annexin V (orb10101) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 36 kD, Observed band size: 36 kD.

Sample: NIH/3T3 (Mouse) Cell Lysate at 30 ug, Lung (Mouse) Lysate at 40 ug, Primary: Anti-Annexin V (orb10101) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 36 kD, Observed band size: 35 kD.

Sample: PANC-1 (Human) Cell Lysate at 30 ug, Primary: Anti-Annexin V (orb10101) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 36 kD, Observed band size: 35 kD.

Tissue/cell: human lung carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-Annexin V Polyclonal Antibody, Unconjugated (orb10101) 1:500, overnight at 4⒉C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/cell: human lung carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-Annexin V Polyclonal Antibody, Unconjugated (orb10101) 1:500, overnight at 4⒉C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/cell: human Neurological glioblastoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-Annexin V Polyclonal Antibody, Unconjugated (orb10101) 1:500, overnight at 4⒉C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/cell: rat lung tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-Annexin V Polyclonal Antibody, Unconjugated (orb10101) 1:500, overnight at 4⒉C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/cell: rat testis tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-Annexin V Polyclonal Antibody, Unconjugated (orb10101) 1:500, overnight at 4⒉C, followed by conjugation to the secondary antibody and DAB staining.