GIT1-S388 Antibody (Center)

• Catalog Number: orb1929279
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB16662
• Tested applications: WB
• Predicted Reactivity: Rat
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, WB: 1:1000
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 84341 Da
• Target: This GIT1-S388 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 366-395 amino acids from the Central region of human GIT1-S388.
• UniProt ID: Q9Y2X7
• NCBI: NP_054749.2, NP_001078923.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: ARF GTPase-activating protein GIT1, ARF GAP GIT1,
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

GIT1-S388 Antibody (Center) western blot analysis in mouse brain tissue lysates (35 ug/lane).This demonstrates the GIT1 antibody detected the GIT1 protein (arrow).

All lanes: Anti-GIT1 Antibody at 1:1000 dilution. Lane 1: U-2OS whole cell lysate. Lane 2: U-251 MG whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 84 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.