General Sphingomyelin (SPH) ELISA Kit

• Catalog Number: orb782007
• Category: Assays and Kits
• Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Sphingomyelin(SPH) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sphingomyelin(SPH).
• Reactivity: All
• Concentration: 400 µg/mL
• Tested applications: ELISA
• Application notes: standard: 400 µg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with SPH protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to SPH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of SPH in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Type: Competitive
• Assay Time: 2.5h
• Range: 6.25-400 µg/mL
• Sensitivity: 1.82 µg/mL
• Sample Types: Serum, plasma and other biological fluids
• Alternative names: SM ELISA Kit
• Research Area: Neuroscience, Signal Transduction
• Note: For research use only
• Expiration Date: Please enquire.