General Sphingomyelin (SPH) ELISA Kit

• Catalog Number: orb1806556
• Category: Assays and Kits
• Description: General Sphingomyelin (SPH) ELISA Kit
• Target: SPH
• Reactivity: All
• Application notes: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Sphingomyelin(SPH) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sphingomyelin(SPH). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sphingomyelin(SPH) in the samples is then determined by comparing the OD of the samples to the standard curve.
• Assay Type: Competitive
• Assay Time: 1.5H
• Standard: 400μg/mL
• Range: 6.25-400μg/mL
• Sensitivity: 1.85 μg/mL
• Sample Types: Serum, plasma and other biological fluids
• Alternative names: SM
• Research Area: Neuroscience, Signal Transduction
• Note: For research use only
• Expiration Date: Please enquire.