GAPDH

• Catalog Number: orb1294414
• Category: Antibodies
• Description: Rabbit polyclonal antibody to GAPDH
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide / encompassing a sequence within the C-terminus region.
• Dilution range: Western Blot 1:5000-1:10000 Immunofluorescence 1:200-1:500 Immunohistochemistry (Paraffin) 1:400-1:600
• Conjugation: Unconjugated
• Target: GAPDH
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Anti-GAPDH antibody - 1/5000 dilution, Lysates at 20 μg per lane. This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294414 overnight at 4°C.

Anti-GAPDH antibody - 1/5000 dilution, Lysates at 20 μg per lane. This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294414 overnight at 4°C.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294414 anti-GAPDH antibody (red) at 1:200 and 4°C.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294414 anti-GAPDH antibody (red) at 1:200 and 4°C.

Immunohistochemical analysis of paraffin embedded mouse testis labeling GAPDH with orb1294414 at 1/400.