GAMT Rabbit pAb Discontinued

SKU: orb247514

Description

Rabbit polyclonal antibody to GAMT

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA, IF, IHC-P, WB
• Dilution Range: WB: 1:500-2000, IHC-P: 1:50-200, IF/ICC: 1:50-200
• Reactivity: Human, Mouse, Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-236 of human GAMT (NP_000147.1).
• Molecular Weight: 29kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti PIG2 antibody, anti CCDS2 antibody, anti TP53I2 antibody, anti HEL-S-20 antibody

Immunofluorescence analysis of A549 cells using GAMT Rabbit pAb (orb247514) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of A549 cells using GAMT Rabbit pAb (orb247514) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of C6 cells using GAMT Rabbit pAb (orb247514) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of C6 cells using GAMT Rabbit pAb (orb247514) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of Hep G2 cells using GAMT Rabbit pAb (orb247514) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of Hep G2 cells using GAMT Rabbit pAb (orb247514) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH-3T3 cells using GAMT Rabbit pAb (orb247514) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH-3T3 cells using GAMT Rabbit pAb (orb247514) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-2 OS cells using GAMT Rabbit pAb (orb247514) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunohistochemistry analysis of paraffin-embedded Human liver using GAMT Rabbit pAb (orb247514) at dilution of 1:500 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Western blot analysis of various lysates using GAMT Rabbit pAb (orb247514) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Quick Database Links

UniProt

Q14353

Chloe Wells 1, Jon Sorgenfrei 1, Sadie L Johnson 1, Devin Albertson 1, Jared Rutter 2 3 4, Steven Andrew Baker Gene delivery of AGAT and GAMT boosts creatine levels in creatine transporter deficiency patient fibroblasts PLoS One, (2025)

PubMed: 40338959

Applications

WB

Reactivity

Human