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Flk-1/VEGFR2 (phospho Tyr1214) rabbit pAb

Catalog Number: orb764189

DispatchUsually dispatched within 5-10 working days
$ 250.00
Catalog Numberorb764189
CategoryAntibodies
DescriptionRabbit polyclonal antibody to Flk-1 (phospho-Tyr1214)
ClonalityPolyclonal
Tested applicationsELISA, IF, IHC-P, WB
ReactivityHuman, Mouse, Rat
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from human VEGFR2 around the phosphorylation site of Tyr1214. AA range:1180-1229
Concentration1 mg/ml
Dilution rangeWestern Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
ConjugationUnconjugated
MW152
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesPBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Alternative namesAnti-KDR antibody, anti-FLK1 antibody, anti-VEGFR2
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NoteFor research use only
Expiration Date12 months from date of receipt.
Flk-1/VEGFR2 (phospho Tyr1214) rabbit pAb

Western Blot analysis of various cells using Phospho-Flk-1 (Y1214) Polyclonal Antibody.

Flk-1/VEGFR2 (phospho Tyr1214) rabbit pAb

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using VEGFR2 (Phospho-Tyr1214) Antibody.

Flk-1/VEGFR2 (phospho Tyr1214) rabbit pAb

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using VEGFR2 (Phospho-Tyr1214) Antibody. The picture on the right is blocked with the phospho peptide.

Flk-1/VEGFR2 (phospho Tyr1214) rabbit pAb

Western blot analysis of lysates from HepG2 cells treated with Na3VO4 0.3nM 40', using VEGFR2 (Phospho-Tyr1214) Antibody. The lane on the right is blocked with the phospho peptide.