FGF2 Antibody

SKU: orb1272539

Description

FGF2 Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: ELISA, NeA, WB
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hFGF-Basic (human Fibroblast Growth Factor-Basic).
• Target: FGF2
• Purification: Anti-hFGF-acidic specific antibody was purified by affinity chromatography employing immobilized hFGF-Basic matrix.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Concentration: batch dependent
• Disclaimer: For research use only

Alternative Names

BFGF, FGFB, FGF-2, HBGF-2, Fibroblast growth factor 2, Basic fibroblast growth factor

To detect Human FGF-basic by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Anti-Human FGF-basic (orb1272538) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human FGF-basic.

To detect Human FGF-basic by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human FGF-basic is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

To detect Human FGF-basic by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human FGF-basic is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentrations are 0.25 ug/ml-0.5 ug/ml for two hours at room temperature. An HRP-labeled polymer detection system was used with DAB chromogen. Heat induced antigen retrieval was performed with a pH 6.0 Sodium Citrate buffer. Optimal concentrations and conditions may vary.

This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentrations are 0.25 ug/ml-0.5 ug/ml for two hours at room temperature. An HRP-labeled polymer detection system was used with DAB chromogen. Heat induced antigen retrieval was performed with a pH 6.0 Sodium Citrate buffer. Optimal concentrations and conditions may vary.

This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentrations are 0.25 ug/ml-0.5 ug/ml for two hours at room temperature. An HRP-labeled polymer detection system was used with DAB chromogen. Heat induced antigen retrieval was performed with a pH 6.0 Sodium Citrate buffer. Optimal concentrations and conditions may vary.

Quick Database Links

Gene Symbol

FGF2

UniProt

P09038

RefSeq:P09038