FBXL5 Antibody (N-term)

SKU: orb1927933

This product can be custom made

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Cell Biology

Images & Validation

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Item 1 of 4

Tested Applications	FC, IHC-P, WB
Dilution Range	WB - 1:1000, IHC-P - 1:100, FC - 1:10-50
Reactivity	Human, Mouse
Predicted Reactivity	Bovine

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Clone No.	RB23907
Immunogen	This FBXL5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 86-115 amino acids from the N-terminal region of human FBXL5. Antigen Region: 86-115 aa.
Target	FBXL5
Molecular Weight	78555 Da
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Form/Appearance	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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F-box/LRR-repeat protein 5, F-box and leucine-rich repeat protein 5, F-box protein FBL4/FBL5, p45SKP2-like protein, FBXL5, FBL4, FBL5, FLR1

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Western blot analysis of FBXL5 Antibody (N-term) in mouse heart tissue lysates (35 ug/lane). FBXL5 (arrow) was detected using the purified Pab.

FBXL5 Antibody (N-term) flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

All lanes: Anti-FBXL5 Antibody (N-term) at 1:1000 dilution. Lane 1: Hela whole cell lysate. Lane 2: U-87 MG whole cell lysate. Lane 3: HepG2 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 79 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemical analysis on paraffin-embedded Human kidney tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.