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F(ab')2 Sheep IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed
Catalog Number: orb348389
| Catalog Number | orb348389 |
|---|---|
| Category | Antibodies |
| Description | F(ab')2 Sheep IgG (H&L) antibody (Peroxidase) |
| Clonality | Polyclonal |
| Species/Host | Donkey |
| Isotype | IgG F(ab')2 |
| Conjugation | HRP |
| Reactivity | Sheep |
| Form/Appearance | Lyophilized |
| Concentration | 1.0 mg/mL |
| Buffer/Preservatives | 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! |
| Purity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Donkey Serum, Sheep IgG and Sheep Serum. No reaction was observed against anti-Pepsin, anti-Donkey IgG F(c) or Chicken, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit and Rat Serum Proteins. |
| Immunogen | Sheep IgG whole molecule |
| Tested applications | ELISA, IHC, WB |
| Dilution range | ELISA: 1:8,000 - 1:40,000, IHC: 1:500 - 1:5,000, WB: 1:2,000 - 1:10,000 |
| Application notes | F(ab')2 Anti-Sheep IgG Peroxidase Conjugated Secondary Antibody reagents are ideal for ELISA, western blotting, Immunohistochemistry, as well as other antibody detection methods. |
| Antibody Type | Secondary Antibody |
| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Alternative names | Donkey F(ab')2 Anti-Sheep IgG Antibody Peroxidase Read more... |
| Note | For research use only |
