F(ab')2 Goat IgG (H&L) Antibody Fluorescein Conjugated Pre-Adsorbed

• Catalog Number: orb348123
• Category: Antibodies
• Description: F(ab')2 Goat IgG (H&L) antibody (FITC)
• Clonality: Polyclonal
• Species/Host: Donkey
• Isotype: IgG F(ab')2
• Conjugation: FITC
• Reactivity: Goat
• Form/Appearance: Lyophilized
• Concentration: 1.0 mg/mL
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Donkey Serum, Goat IgG and Goat Serum. No reaction was observed against anti-Pepsin, anti-Donkey IgG F(c) or Chicken, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit and Rat Serum Proteins.
• Immunogen: Goat IgG whole molecule
• Tested applications: DOT, FC, FLISA, IF
• Dilution range: FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000
• Application notes: F(ab')2 Anti-Goat IgG Fluorescein Antibody has been tested by dot blot and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
• Antibody Type: Secondary Antibody
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Alternative names: Donkey F(ab')2 Anti-Goat IgG Antibody Fluorescein
• Note: For research use only

Dot Blot results of Donkey F(ab')2 Anti-Goat IgG Antibody Fluorescein Conjugate. Dots are Goat IgG: (1) 100 ng, (2) 33.3 ng, (3) 11.1 ng, (4) 3.70 ng, (5) 1.23 ng. Primary Antibody: none. Secondary Antibody: Donkey F(ab')2 Anti-Goat IgG Antibody FITC at 1 ug/ml in orb348637 1hr RT.