F(ab')2 Cat IgG (H&L) Antibody Peroxidase Conjugated

• Catalog Number: orb348085
• Category: Antibodies
• Description: F(ab')2 Cat IgG (H&L) antibody (Peroxidase)
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG F(ab')2
• Conjugation: HRP
• Reactivity: Feline
• Form/Appearance: Lyophilized
• Concentration: 1.0 mg/mL
• Buffer/Preservatives: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Cat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Cat IgG and Cat Serum. No reaction was observed against anti-Pepsin or anti-Rabbit IgG F(c).
• Immunogen: Anti-Cat IgG (H&L) was produced by repeated immunization with Cat IgG whole molecule in rabbit.
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:100,000, IHC: 1:500 - 1:5,000, WB: 1:5,000 - 1:100,000
• Application notes: F(ab')2 anti-Cat IgG Peroxidase conjugated Antibody has been tested by ELISA and is assayed against 1.0 ug of Cat IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:8,000 of the reconstitution concentration is suggested for this product.
• Antibody Type: Secondary Antibody
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Alternative names: Rabbit F(ab')2 Anti-Cat peroxidase Conjugated Anti
• Note: For research use only
• Expiration Date: 12 months from date of receipt.