Fab Guinea Pig IgG (H&L) Antibody Biotin Conjugated

SKU: orb348443

Description

Fab Guinea Pig IgG (H&L) antibody (Biotin)

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Reactivity: Guinea pig
• Application Notes:
  Suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

Key Properties

• Antibody Type: Secondary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG Fab
• Immunogen: Guinea Pig IgG whole molecule
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Guinea Pig IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin and anti-Rabbit Serum. No reaction was observed against anti-Papain or anti-Rabbit IgG F(c).
• Conjugation: Biotin

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 0.5 mg/mL
• Disclaimer: For research use only

Alternative Names

Rabbit Fab Anti-Guinea Pig IgG Biotin Conjugated Antibody, Rabbit Fab Fragment Anti-Guinea Pig IgG Antibody Biotin Conjugation

(B) Maximum projection of fluorescence images of QD-labeled receptors diffusing in the membrane during the experiment and showing the shape of a thin and a large GC. Scale bar 10 µm.