ESR1/ER Antibody (Center)

SKU: orb1936599

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Cancer Biology

Images & Validation

• Tested Applications: IF, IHC-P, WB
• Dilution Range: IF - 1:10-50, WB - 1:2000, IHC-P - 1:100
• Reactivity: Human, Mouse
• Predicted Reactivity: Equine, Porcine, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB33807
• Immunogen: This ESR1/ER antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 246-273 amino acids from the Central region of human ESR1/ER. Antigen Region: 246-273 aa.
• Target: ESR1
• Molecular Weight: 66216 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Estrogen receptor, ER, ER-alpha, Estradiol receptor, Nuclear receptor subfamily 3 group A member 1, ESR1, ESR, NR3A1

All lanes: Anti-ESR1/ER Antibody (Center) at 1:2000 dilution. Lane 1: MCF-7 whole cell lysate. Lane 2: T47D whole cell lysate. Lane 3: ZR-75-1 whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 66 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-ESR1/ER Antibody (Center) at 1:2000 dilution + Mouse testis whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 66 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Confocal immunofluorescent analysis of ESR1/ER Antibody (Center) with ZR-75-1 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

Immunohistochemical analysis on paraffin-embedded Human breast carcinoma tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary Antibody.

Immunohistochemical analysis on paraffin-embedded Human uterus tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary Antibody.

Quick Database Links

Gene Symbol

ESR1

UniProt

P03372

RefSeq (Protein):NP_000116.2, NP_001116214.1, NP_001116212.1, NP_001116213.1