E-cadherin (Extracellular) Rabbit Polyclonal Antibody

SKU: orb1294382

Description

Rabbit polyclonal antibody to E-cadherin antibody (Extracellular domain)

Images & Validation

• Tested Applications: IF, IHC, WB
• Dilution Range: Western Blot 1:500-1:1000, Immunofluorescence 1:200-1:500, Immunohistochemistry (Paraffin) 1:100-1:200
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide / Extracellular domain corresponding to Human E-cadherin
• Target: E-cadherin (Extracellular)
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

All lanes: Anti-E cadherin antibody at 1/500 dilution, Lysates/proteins at 60 µg per lane. This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked with 3% BSA for an hour before being incubated with orb1294382 overnight at 4°C.

All lanes: Anti-E cadherin antibody at 1/500 dilution, Lysates/proteins at 60 µg per lane. This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked with 3% BSA for an hour before being incubated with orb1294382 overnight at 4°C.

Anti-E cadherin antibody at 1/500 dilution, Lysates/proteins at 60 µg per lane. This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked with 3% BSA for an hour before being incubated with orb1294382 overnight at 4°C.

Immunofluorescence of paraffin-embedded sections. Cell: mouse colon, Green: E-cadherin (orb1294382): 1-200, Anti-rabbit 488: 1-300.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling E-cadherin with orb1294382 at 1/100.

Immunohistochemical analysis of paraffin embedded Human Liver carcinoma tissue labeling E-cadherin with orb1294382 at 1/100.

Quick Database Links

Gene Symbol

E-cadherin (Extracellular)