E Cadherin (CDH1) Antibody (N-term)

• Catalog Number: orb1935497
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB13430
• Tested applications: FC, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:2000, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 97456 Da
• Target: This E Cadherin (CDH1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 160-189 amino acids from the N-terminal region of human E Cadherin (CDH1).
• UniProt ID: P12830
• NCBI: NP_004351.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Cadherin-1, CAM 120/80, Epithelial cadherin, E-cad
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometric analysis of NCI-H292 cells using E Cadherin (CDH1) Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

All lanes: Anti-CDH1 Antibody (N-term) at 1:2000 dilution. Lane 1: 293 whole cell lysate. Lane 2: A431 whole cell lysate. Lane 3: A549 whole cell lysate. Lane 4: DU145 whole cell lysate. Lane 5: MCF-7 whole cell lysate. Lane 6: T47D whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 97 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.