Donkey IgG (H&L) Antibody Biotin Conjugated

• Catalog Number: orb347996
• Category: Antibodies
• Description: Donkey IgG (H&L) antibody (Biotin)
• Clonality: Polyclonal
• Species/Host: Goat
• Isotype: IgG
• Conjugation: Biotin
• Reactivity: Donkey
• Form/Appearance: Lyophilized
• Concentration: 2.0 mg/mL
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Purity: Anti-Donkey IgG (H&L) was prepared from monospecific antiserum by immunoaffinity chromatography using Donkey IgG coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Donkey IgG and Donkey Serum.
• Immunogen: Donkey IgG whole molecule
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Application notes: Anti-Donkey IgG (H&L) has been assayed against 1.0 ug of Donkey IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:40,000 to 1:160,000 of the reconstitution concentration is suggested for this product. Specific conditions for reactivity should be optimized by the end user.
• Antibody Type: Secondary Antibody
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Alternative names: goat anti-Donkey IgG Antibody biotin conjugation,
• Note: For research use only
• Expiration Date: 12 months from date of receipt.