Cytokeratin 20 antibody

SKU: orb2276563

Description

Cytokeratin 20 antibody validated for immunohistochemistry on 76 different Normal Tissues

Images & Validation

• Tested Applications: IHC
• Dilution Range: 1:100-1:200
• Reactivity: Human
• Application Notes:
  Positive Control: Colon mucosa: Staining should be strong at the surface and at least moderate at the basis of krypts. Negative Control: Tonsil: All cells must stain negative. Cellular Localization: Cytoplasmic Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

Key Properties

• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: MSVA-620R
• Immunogen: Recombinant fragment of human KRT20 protein (around aa 196-323) (exact sequence is proprietary)
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CK20; Cytokeratin-20; K20; KA20; Keratin 20; keratin 20, type I; Keratin type I cytoskeletal 20; Keratin-20; KRT20

In the colon mucosa CK20 staining is strongest at the surface epithelium and decreased towards the crypt base.

CK20 staining is absent in the kidney.

Stomach, antrum: In the stomach, CK20 immunostaining is preferentially seen in the cells of the surface epithelium while staining is absent in the deep glands.

Quick Database Links

UniProt

P35900