KRT20 Antibody

SKU: orb1410421

Description

Rabbit monoclonal antibody to Cytokeratin 20 (Colorectal Epithelial Marker)

Research Area

Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: IHC, WB
• Reactivity: Human

Key Properties

• Antibody Type: Recombinant Antibody
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: KRT20/4879R
• Immunogen: Recombinant fragment of human KRT20 protein (around aa 196-323) (exact sequence is proprietary)
• Target: KRT20
• Source/Expression System: HEK293 cells
• Molecular Weight: 46kDa
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CK20; Cytokeratin-20; K20; KA20; Keratin 20; keratin 20, type I; Keratin type I cytoskeletal 20; Keratin-20; KRT20

IHC analysis of formalin-fixed, paraffin-embedded human brain. stained with OLIG2/6695R at 2 ug/ml in PBS for 30 min RT. Inset: PBS instead of primary; secondary antibody negative control.

IHC analysis of formalin-fixed, paraffin-embedded human colon. stained with KRT20/4879R at 2 ug/ml in PBS for 30 min RT. HIER: Tris/EDTA, pH9.0, 45 min. 2°C: HRP-polymer, 30 min. DAB, 5 min.

SDS-PAGE Analysis of Purified CK20 Recombinant Rabbit Monoclonal Antibody (KRT20/4879R). Confirmation of Purity and Integrity of Antibody.

Western Blot Analysis of HT-29 cell lysate using Cytokeratin 20 Recombinant Rabbit Monoclonal Antibody (KRT20/4879R).

Quick Database Links

Gene Symbol

KRT20

UniProt

P35900