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| Catalog Number | orb1410421 |
|---|---|
| Category | Antibodies |
| Description | Rabbit monoclonal antibody to Cytokeratin 20 (Colorectal Epithelial Marker) |
| Target | KRT20 |
| Clonality | Monoclonal |
| Species/Host | Rabbit |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Form/Appearance | 200ug/ml of Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml. |
| Immunogen | Recombinant fragment of human KRT20 protein (around aa 196-323) (exact sequence is proprietary) |
| UniProt ID | P35900 |
| MW | 46kDa |
| Tested applications | IHC, WB |
| Antibody Type | Recombinant Antibody |
| Clone Number | KRT20/4879R |
| Expression System | HEK293 cells |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | CK20; Cytokeratin-20; K20; KA20; Keratin 20; kerat Read more... |
| Research Area | Stem Cell & Developmental Biology |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |

IHC analysis of formalin-fixed, paraffin-embedded human brain. stained with OLIG2/6695R at 2 ug/ml in PBS for 30 min RT. Inset: PBS instead of primary; secondary antibody negative control.

IHC analysis of formalin-fixed, paraffin-embedded human colon. stained with KRT20/4879R at 2 ug/ml in PBS for 30 min RT. HIER: Tris/EDTA, pH9.0, 45 min. 2°C: HRP-polymer, 30 min. DAB, 5 min.

SDS-PAGE Analysis of Purified CK20 Recombinant Rabbit Monoclonal Antibody (KRT20/4879R). Confirmation of Purity and Integrity of Antibody.

Western Blot Analysis of HT-29 cell lysate using Cytokeratin 20 Recombinant Rabbit Monoclonal Antibody (KRT20/4879R).
IHC, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
IHC | |
Human | |
Rabbit | |
Monoclonal | |
Unconjugated |
IHC, WB | |
Human | |
Rabbit | |
Monoclonal | |
Unconjugated |
IHC, WB | |
Human | |
Rabbit | |
Monoclonal | |
Unconjugated |
WB | |
Human | |
Rabbit | |
Monoclonal | |
Unconjugated |
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